The Effect that Phosphoric Acid, Calcium Carbonate, and Temperature has on the Growth and Decay of Bone Marrow Colonies
The objective of the experiment was to determine whether a difference in the number of bone marrow colonies would occur when the bone marrow colonies were subjected to phosphoric acid, calcium carbonate, and differing temperatures. The experiment was very simplistic in concept: live bone marrow was put into a Petri dish, and then a picture was taken so that the researchers could count the number of colonies growing in the dish at the commencement of the experiment. Every day for a week, the three factors were adjusted in the Petri dishes, and the change in the number of colonies was recorded so that the researchers could see if there was any change in the number of bone marrow colonies. The percent difference in bone marrow colonies from start to finish was calculated and these values were analyzed using a three-factor Design of Experiment. The Phosphoric Acid showed a negative effect causing the number of colonies to decline; whereas, the Calcium Carbonate was conducive to the bone marrow colonies and the number of colonies increased. Temperature was slightly helpful to the increasing of the number of bone marrow colonies. The experiment showed a possible interaction between Phosphoric Acid and Calcium Carbonate as affecting each other during the experiment. There was no interaction of the Calcium Carbonate or Phosphoric Acid with the temperature variable.
Research Done By:
Tyler Fish
L'anse Creuse North High School
Christopher Hopper
L'anse Creuse North High School
Warren Consolidated Schools